Brand: Thermo ScientificTM OxoidTM Oxoid Anaerobe Basal Agar is used for growth of fastidious and other anaerobes. Also available N-S Anaerobe Selective Supplement, Part No. SR0107B, for use for nonsporforming anaerobes and G-N Anaerobe Selective Supplement, Part No. SR0108B, for use for gram-negative anaerobes. Anaerobe Basal Agar, Oxoid Composition Typical Formula * gm/litre Peptone 16.0 Yeast extract 7.0 Sodium chloride 5.0 Starch 1.0 Dextrose 1.0 Sodium pyruvate 1.0 Arginine 1.0 Sodium succinate 0.5 L-cysteine HCl 0.25 Sodium bicarbonate 0.4 Ferric pyrophosphate 0.5 Haemin 0.005 Vitamin K 0.0005 Dithiothreitol 0.25 Agar 12.0 pH 6.8 ± 0.2 @ 25°C Anaerobe Basal Agar, Oxoid Preparation: Suspend 46g in 1 litre of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add 5-10% sterile Defibrinated Horse Blood SR0050. Storage conditions and Shelf life Store the dehydrated medium at 10-30°C and use before the expiry date on the label. Store the prepared plates of medium for upto 3 weeks at 2-8°C.
